Ecotoxicology studies are performed to assess the impact of the contamination of compounds on various ecosystems. The studies are conducted by following the Principles of Good laboratory Practices. The studies are conducted in accordance with the standard operating procedures of test facility and mutually agreed study plans and amendments. Instruments required in the experiments are well equipped and procured from the authenticated vendors having ISO/NABL certification. Internal as well as external calibrations are also performed at regular intervals. The ecotoxicology studies are supported by the in-house Method Validation prior performing the test. 000+ ecotoxicology studies were performed and submitted to regulatory authorities.
The aquatic testing at our facility includes the following studies:
OECD 201 - Freshwater Alga and Cyanobacteria, Growth Inhibition Test
The ecotoxicology professionals at
ESSEM Compliance Solutions Private Limited surrounds with a broad range of non-clinical studies with freshwater fish, zooplankton and algae.
Alga, Growth Inhibition Tests are performed as per the OECD 201 guideline to assess the effect of test item on freshwater algae (
Psuedokirchneriella subcapitata) for a period of 72 hours. It refers to the determination of 50% inhibition of growth rate expressed as the logarithmic increase in biomass (average specific growth rate) during the exposure period denoted as ErCx.
Alga, Growth Inhibition studies are assisted by the Analytical Chemistry department of
ESSEM Compliance Solutions Private Limited for method development and validation, characterization of test item, homogeneity, stability and dose concentration verification in test media as per the regulatory requirements.
OECD 202 - Daphnia sp. Acute Immobilization Test
The ecotoxicology professionals at
ESSEM Compliance Solutions Private Limited surrounds with a broad range of non-clinical studies with freshwater fish, zooplankton and algae.
Daphnia sp. Acute Immobilization Tests are performed as per the OECD 202 guideline to assess the acute toxicity of test item when exposed to zooplankton (
Daphnia magna) for a period of 48 hours. It refers to the determination of 50% effective concentration at which daphnids are found to be immobile. The results are analyzed in order to calculate the EC
50 at 48 hours although; EC
50 at 24 hours is optional.
Daphnia sp. Acute Immobilization studies are assisted by the Analytical Chemistry department of
ESSEM Compliance Solutions Private Limited for method development and validation, characterization of test item, homogeneity, stability and dose concentration verification in test media as per the regulatory requirements.
OECD 203 - Fish, Acute Toxicity Test
The ecotoxicology professionals at
ESSEM Compliance Solutions Private Limited surrounds with a broad range of non-clinical studies with freshwater fish, zooplankton and algae.
Fish, Acute Toxicity Tests are performed as per the OECD 203 guideline to assess the acute toxicity of test item when exposed to freshwater fish (
Danio rerio) for a period of 96 hours. It refers to the determination of 50% lethal concentration at which fish are found to be dead.
Fish, Acute Toxicity studies are assisted by the Analytical Chemistry department of
ESSEM Compliance Solutions Private Limited for method development and validation, characterization of test item, homogeneity, stability and dose concentration verification in test media as per the regulatory requirements.
OECD 210 - Fish, Early - Life Stages Toxicity Test
Along with the acute toxicity studies, the ecotoxicology professionals at
ESSEM Compliance Solutions Private Limited are expertise in chronic studies as well.
When the results of Fish, Acute Toxicity Test found to be toxic, chronic data are required by the regulatory authority for registration of test item. Tests with the Early Life - Stages of fish are intended to define the lethal and sub lethal effects of test item on the stages and species tested.
Fish, Early - Life Stages Toxicity Tests are performed as per the OECD 210 guideline to assess the chronic toxicity of test item when exposed to the newly fertilized eggs of freshwater fish (
Danio rerio) for a period of 30 days post hatch. At the end of the exposure, lethal and sub lethal effects are assessed and compared with control to determine NOEC, LOEC and EC
50.
Fish, Early - Life Stages Toxicity studies are assisted by the Analytical Chemistry department of
ESSEM Compliance Solutions Private Limited for method development and validation, characterization of test item, homogeneity, stability and dose concentration verification in test media as per the regulatory requirements.
OECD 211 - Daphnia Magna Reproduction Test
Along with the short term immobilization studies, the ecotoxicology professionals at
ESSEM Compliance Solutions Private Limited are highly experienced in long term studies as well.
When the results of Acute Immobilization Test found to be toxic, chronic data are required by the regulatory authority for registration of test item.
Daphnia magna Reproduction Tests are developed to determine the toxicity of test item on the reproductive output of the test species.
Daphnia magna Reproduction Tests are performed as per the OECD 211 guideline to assess the chronic toxicity of test item when exposed to the aquatic invertebrate (
Daphnia magna) for a period of 21 days. The toxic effect of the test item on reproductive output is expressed as ECx calculated by the concentration response curve using various statistical methods (Probit/ StatPlus/ GraphPad Prism) along with the NOEC, LOEC values.
Daphnia magna Reproduction studies are assisted by the Analytical Chemistry department of
ESSEM Compliance Solutions Private Limited for method development and validation, characterization of test item, homogeneity, stability and dose concentration verification in test media as per the regulatory requirements.
